Frustrated teachers and public sector workers will walk off the job in Hobart and Launceston amid a worsening wages dispute with the state government.
Unions expect thousands of public staff to take part in stop-work meetings on Wednesday.
The two-day strikes began in the northwest on Tuesday, where teachers left work early and forced more than 30 schools to close.
"We understand that it is a disruption for parents, but the parents who take their children into school every day see the work their teachers and support staff are doing," Australian Education Union (AEU) state manager Roz Madsen said.
Public bus drivers in the northwest also took industrial action, affecting about 50 services around Burnie.
Stop-work meetings will be held in Launceston in the morning and Hobart in the afternoon.
About 100 schools are expected to be affected across Tasmania.
Public sector workers have been at loggerheads with the Liberal government for the best part of a year over pay and conditions.
The AEU Tasmania branch on Monday rejected the government's pay offer of seven per cent over three years.
The AEU wants a pay rise of three per cent per year.
The government's latest pay offer is 0.25 per cent higher than its previous offer.
© AAP 2019